Product Details:
|
Package: | 96 Tests/kit | Expiration Date: | 12 Months |
---|---|---|---|
Storage: | -20±5℃ | Applicable: | Micgene 244/244 IVD(BIO-KEY) |
LoD: | 1000 Copies/mL | CV: | ≤5% |
High Light: | CE RT PCR Detection Kit,Trichomonas Vaginalis PCR Detection Kit,RT PCR Detection Kit Lyophilized |
Intended use:
Principle:
Main components:
No. | Components | BIK-QL-H0020 |
1 |
PCR Enzyme Mix(Lyophilized) |
96 tests/bottle |
2 | TV Primer-Probe Mix | 100 μL/vial |
3 | Enzyme Mix Buffer (5×) | 400 μL/vial |
4 | Positive Control | 100 μL/tube |
5 | Negative Control | 100 μL/tube |
6 | RNase-free water | 1mL/tube |
7 | Paraffin oil | 1.5mL/tube*2 |
Specimen requirements:
1. Specimen type
Urine or female vaginal secretions swab samples.
2. Specimen Preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
PCR Amplification condition:
PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 95℃ | 3min |
2 | 40 | 95℃ | 10s |
60℃ | 30s collect fluorescent |
Instrument detection channel selection: FAM and HEX/VIC.
Quality control:
Ct value | |
Negative Control | No Ct |
Positive Control | ≤30 |
IC of samples | ≤38 |
Note: Negative Control, Positive Control and IC of samples must be performed correctly, otherwise the sample results is invalid.
Data analysis and interpretation:
The following sample results are possible:
Ct value | Result Analysis | |
1# | No Ct | negative |
2# | ≤38 | Positive |
3# | 38~40 | Re-test; if it is still 38~40, report as 2# |
Operation details please check from IFU!
Contact Person: Tracy
Tel: +8618802073256