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Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized

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Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized

Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized
Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized

Large Image :  Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: CE
Model Number: BIK-QL-H0020S1
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
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Operation Manual Trichomonas Vaginalis TV RT PCR Detection Kit Lyophilized

Description
Package: 24 Tests/kit Expiration Date: 12 Months
Storage: -20±5℃ Applicable: Micgene 244/244 IVD（BIO-KEY）
LoD: 1000 Copies/mL CV: ≤5%
High Light:

Trichomonas Vaginalis RT PCR Kit

,

TV RT PCR Detection Kit

,

24Tests/kit STD STI Test Kit

Trichomonas vaginalis (TV) RT-PCR Detection Kit (Lyophilized) Operation Manual
--24 tests/kit

 
Intended use:
 

By using real time PCR systems, Trichomonas vaginalis (TV) real time PCR kit is used for the in vitro qualitative detection of TV. It can be used for the laboratory diagnosis and monitoring of TV infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Principle:
 

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Trichomonas vaginalis (TV). The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of TV through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


 
Main components:

 

No. Components

BIK-QL-H0020S1

1

PCR Enzyme Mix(Lyophilized)

24tests/kit
2 Positive Control 100 μL/tube
3 Negative Control 100 μL/tube
4 RNase-free water 1mL/tube
5

Paraffin oil

1.5mL/tube

  

Note: Do not mix the components from different batches for detection. The Positive Control of TV and internal control were constructed artificially, and they were not infectious.

 

Specimen requirements:
 

1. Specimen type

Urine or female vaginal secretions swab samples.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

 

 

PCR Amplification condition:

 
PCR amplification according to the following procedures:
 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 40 95℃ 10s
60℃ 30s collect fluorescent

 

Instrument detection channel selection: FAM and HEX/VIC.
 
Quality control:
 

  Ct value
Negative Control No Ct
Positive Control ≤30
IC of samples ≤38


Note: Negative Control, Positive Control and IC of samples must be performed correctly, otherwise the sample results is invalid.
 

Performance indexes of product:

 

  • Accuracy:Use national reference products or enterprise positive/negative reference products for testing, and the results are all positive/negative.
  • Detection limitation:1000 copies/mL.
  • Precision: The coefficient of variation (CV) of Ct values is≤5%.
  • Specificity: The detection results of this kit have no cross reaction with Urealytic Mycoplasma, Mycoplasma hominis, Ureaplasma urealyticum, Mycoplasma pneumoniae,Neisseria gonorrhoeae,Chlamydia trachomatis,herpes simplex virus type Ⅱ,Mycoplasma genitalium.

 Operation details please check from IFU!

Tag:

TV STD STI Test Kit,

Operation Manual RT PCR Kit,

Lyophilized RT PCR Detection Kit

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Tracy

Tel: +8618802073256

Send your inquiry directly to us (0 / 3000)

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