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Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit

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Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit

Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit
Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit

Large Image :  Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: ISO 13485,CE
Model Number: BIK-QL-H004 / BIK-QL-H004S
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 kits per day

Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit

Description
Package: 48 T/ Kit(single Tube) / 96 T/kit (box) Expiration Date: 12 Months
Storage: -20±5℃ Applicable: Micgene 244/244 IVD(BIO-KEY)
Detection Limit: 500 Copies/mL CV: ≤5%
High Light:

Lyophilized Respiratory PCR Test Kit

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Respiratory PCR Test Kit Fluorescent Taqman

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Taqman Probe Rt Pcr Rapid Test Kit

Multiple Respiratory viral RT-PCR test kit (Lyophilized)

 
Intended use:
 

This kit is used for in vitro qualitative detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae from patients with respiratory tract infection. The test results are for clinical reference only, it cannot be used alone as the basis for the diagnosis or exclusion of cases

 

Test Principle:


The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae, the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different fluorescent groups. On the fluorescence quantitative PCR instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae through the change of fluorescent signal.The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae, the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different fluorescent groups. On the fluorescence quantitative PCR instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae through the change of fluorescent signal.The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.

Biokey Multiple Respiratory PCR testing solution.p...


Product components:

 

1. BIK-QL-H004:

No. Components Amount
1 PCR Enzyme Mix (Lyophilized) 96 tests/bottle
2 Primer-Probe Mix 1 (SARS-CoV-2/Influenza A+B ) 50 μL/vial
3 Primer-Probe Mix 2 (RSV/Adenovirus/M.pneumoniae) 50 μL/vial
4 Enzyme Mix Buffer (5×) 400 μL/vial
5 Positive Control 100 μL/tube
6 Negative Control 100 μL/tube
7 RNase-free water 1mL/tube
8 Paraffin oil 1.5mL/tube*2

 

2. BIK-QL-H004S:

No. Components Amount
1 1 (SARS-CoV-2/Influenza A+B Mix (Lyophilized)) 24 tests/kit
2 2 (RSV/Adenovirus/M.pneumoniae Mix (Lyophilized)) 24 tests/kit
3 Positive Control 100 μL/tube
4 Negative Control 100 μL/tube
5 RNase-free water 1mL/tube
6 Paraffin oil 1.5mL/tube

 

Note: Do not mix the components from different batches for detection. The Positive Control of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae and internal control were constructed artificially, and they were not infectious.

 

Reagent preparatation:

 

(1) Preparation of [BIK-QL-H004] PCR mix as follows:

① Master Mix Preparation:Take out the components from the box and let it thaw at room temperature until equilibrated. Resuspend the Lyophilized Enzyme Mix in 400μL Enzyme Mix Buffer. Add 1mL RNase-free water and gently pipette up and down. Avoid generating air bubbles. Wash the wall of tube by pipetting to prevent lyophilized powder from remaining. Place the tube aside for 30 min.

 

②Reaction Mix Preparation: Refer to the tables below to prepare the reaction mix:

Ⅰ. SARS-CoV-2/Influenza A+B reaction mix:

1×volume required
Resuspended master mix 14 μL
Primer-Probe Mix 1 1 μL
Total volume 15 μL

 

 

Ⅱ. RSV/Adenovirus/M.pneumoniae reaction mix:

1×volume required
Resuspended master mix 14 μL
Primer-Probe Mix 2 1 μL
Total volume 15 μL

 

 Multiply the numbers according to the number of tests.

 

Lyophilization package :

 

Lyophilized Multiple Respiratory PCR Test Kit Fluorescent Taqman Probe Pcr Detection Kit 0

 

 

Operation details please check from IFU

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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