Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized

Name: Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized
Brand: Biokey
SKU: BIK-QL-H0022S1
Price: 1.00 USD
Availability: InStock
Rating: 4.7 (195 reviews)

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Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized

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Product Details:

Place of Origin:	Guangzhou China
Brand Name:	Biokey
Certification:	CE
Model Number:	BIK-QL-H0022S1

Payment & Shipping Terms:

Minimum Order Quantity:	We can produce liquid and lyophilized kits
Price:	USD
Packaging Details:	Carton package
Delivery Time:	Depending on order quantity
Payment Terms:	L/C, T/T, Western Union
Supply Ability:	100,000 per day

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Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized

Description

Package:	24 Tests/kit	Expiration Date:	12 Months
Storage:	-20±5℃	Applicable:	Micgene 244/244 IVD（BIO-KEY）
LoD:	1×1000 Copies/ml	CV:	≤5%
High Light:	Ureaplasma Parvum STD STI Test Kit , Ureaplasma Urealyticum PCR Detection Kit , 24Tests/Kit STD STI Test Kit

UP+UU real-time PCR detection kit (lyophilized)

--24 tests/kit

Intended use:

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Principle:

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

UP+UU product lyophilization pacakage:

Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized 0

Main components:

NO.	Components	BIK-QL-H0022S1
1	PCR Enzyme Mix(Lyophilized)	24 tests/kit
2	Positive Control	100 μL/tube
3	Negative Control	100 μL/tube
4	RNase-free water	1mL/tube
5	Paraffin oil	1.5mL/tube

Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.

Specimen requirements:

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

PCR Amplification condition:

PCR amplification according to the following procedures:

Step	Cycle number	Temperature	Time
1	1	95℃	3min
2	40	95℃	10s
2	40	60℃	30s collect fluorescent

Instrument detection channel selection: FAM and HEX/VIC.

Quality control:

Negative Control: None of the FAM, VIC/HEX & Internal Control (ROX) channels have a Ct value or Ct ＞ 40;

Positive Control: FAM, VIC/HEX & Internal Control (ROX) channels Ct ≤ 30;

The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.

Data analysis and interpretation:

	FAM (UP)	VIC/HEX (UU)	ROX (IC)	Interpretation test results
1	-	-	-	Result invalid,test again
2	+	-	+/-	UP(+)
3	-	+	+/-	UU(+)
4	+	+	+/-	UP and UU(+)
5	-	-	+	Negative(-)

NOTE: ‘+’ is positive, ‘-’ is negative.

Operation details please check from IFU!

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Contact Person: Ms. Lisa

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