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Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized

Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-QL-W051
Package:
Single Tube, Lyophilized Powder
Fluorescent Channel:
FAM
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Fish Virus Nucleic Acid Detection Kit

,

PCR Lyophilized Nucleic Acid Detection Kit

Product Description

Tilapia Lake Virus Nucleic Acid Detection Kit (Real time PCR, Lyophilized)


 
Intended use:

 

This kit is used for the specific detection of the Tilapia Lake virus (TiLV) in liver, spleen, kidney, and brain tissue samples of silk perch, Chinese mitten carp, Chinese giant salamander, and zebrafish.
 
 

Principle:

 

The kit synthesizes cDNA strands complementary to the RNA template using RNA as the template and primers as the starting point under the action of efficient reverse transcriptase. The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Tilapia Lake Virus.

 

The Real Time PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of Tilapia Lake Virus through the change of fluorescence signal.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes/kit
Positive control 100ul/tube
Negative control 100ul/tube
reconstitution 600ul/tube

 

Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized 0

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50°C 20 min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-QL-W051
Package:
Single Tube, Lyophilized Powder
Fluorescent Channel:
FAM
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Fish Virus Nucleic Acid Detection Kit

,

PCR Lyophilized Nucleic Acid Detection Kit

Product Description

Tilapia Lake Virus Nucleic Acid Detection Kit (Real time PCR, Lyophilized)


 
Intended use:

 

This kit is used for the specific detection of the Tilapia Lake virus (TiLV) in liver, spleen, kidney, and brain tissue samples of silk perch, Chinese mitten carp, Chinese giant salamander, and zebrafish.
 
 

Principle:

 

The kit synthesizes cDNA strands complementary to the RNA template using RNA as the template and primers as the starting point under the action of efficient reverse transcriptase. The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Tilapia Lake Virus.

 

The Real Time PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of Tilapia Lake Virus through the change of fluorescence signal.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes/kit
Positive control 100ul/tube
Negative control 100ul/tube
reconstitution 600ul/tube

 

Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
Tilapia Lake Fish Virus Nucleic Acid Detection Kit Real Time PCR Lyophilized 0

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50°C 20 min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

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