MOQ: | We can produce liquid and lyophilized kits |
Price: | USD |
Standard Packaging: | Carton package |
Delivery Period: | Depending on order quantity |
Payment Method: | L/C, T/T, Western Union |
Supply Capacity: | 100,000 per day |
Nocardia seriolae Nucleic Acid Detection Kit (Real time PCR-Fluorescent Probe method)
Intended use:
The kit is used for the epidemiological analysis of Nocardia seriolae in fish viscera tissue samples
Principle:
In this kit, the specific fragment of N.seriolae is used to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Therefore, the quenched 3 '-end group was separated and the fluorescence was emitted. The fluorescence PCR instrument was used to detect Nocardia seriolae in a completely closed reaction system
Main components:
Component | Specification |
PCR reaction liquid | 24 tubes |
Positive control | 1 tube |
Negative control | 1 tube |
1. Sample type
Gills, spleen, kidney and other pathological tissues.
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 94℃ | 3min |
2 | 45 | 94℃ | 10s |
60℃ | 20s collect fluorescent |
FAM was selected as the detection channel.
Quality control:
The results of interpretation
Results | Ct Value |
Positive | Ct≤38 |
Negative | No Ct value or Ct value≥45 |
Gray area | 38<Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Product picture:
Operation details please check from IFU!
MOQ: | We can produce liquid and lyophilized kits |
Price: | USD |
Standard Packaging: | Carton package |
Delivery Period: | Depending on order quantity |
Payment Method: | L/C, T/T, Western Union |
Supply Capacity: | 100,000 per day |
Nocardia seriolae Nucleic Acid Detection Kit (Real time PCR-Fluorescent Probe method)
Intended use:
The kit is used for the epidemiological analysis of Nocardia seriolae in fish viscera tissue samples
Principle:
In this kit, the specific fragment of N.seriolae is used to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Therefore, the quenched 3 '-end group was separated and the fluorescence was emitted. The fluorescence PCR instrument was used to detect Nocardia seriolae in a completely closed reaction system
Main components:
Component | Specification |
PCR reaction liquid | 24 tubes |
Positive control | 1 tube |
Negative control | 1 tube |
1. Sample type
Gills, spleen, kidney and other pathological tissues.
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 94℃ | 3min |
2 | 45 | 94℃ | 10s |
60℃ | 20s collect fluorescent |
FAM was selected as the detection channel.
Quality control:
The results of interpretation
Results | Ct Value |
Positive | Ct≤38 |
Negative | No Ct value or Ct value≥45 |
Gray area | 38<Ct<45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Product picture:
Operation details please check from IFU!