|Specimen:||Fish Fry Or Fish Heart, Intestine, Spleen, Kidney And Other Visceral Tissue Tissue Samples.||Applicable:||Micgene 242 / 244 、ABI QuantStudio5|
|Mini Limit:||10 Copies/μL||Positive:||Ct≤38|
PCR Aquaculture Test Kit,
Aquaculture Test Kit Nocardia Seriolae,
Nocardia Seriolae Nucleic Acid Detection Kit ISO
Nocardia seriolae Nucleic Acid Detection Kit (Real time PCR-Fluorescent Probe method)
The kit is used for the epidemiological analysis of Nocardia seriolae in fish viscera tissue samples
In this kit, the specific fragment of N.seriolae is used to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclide activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Therefore, the quenched 3 '-end group was separated and the fluorescence was emitted. The fluorescence PCR instrument was used to detect Nocardia seriolae in a completely closed reaction system
|PCR reaction liquid||24 tubes|
|Positive control||1 tube|
|Negative control||1 tube|
1. Sample type
Gills, spleen, kidney and other pathological tissues.
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence detection (PCR detection area):
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
|60℃||20s collect fluorescent|
FAM was selected as the detection channel.
The results of interpretation
|Negative||No Ct value or Ct value≥45|
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Product performance index:
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
Operation details please check from IFU!
Contact Person: Ms. Lisa