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PCR Fluorescent Strep Test Kit Aquaculture Test Kit Iniae Septicaemia Virus

PCR Fluorescent Strep Test Kit Aquaculture Test Kit Iniae Septicaemia Virus

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W025
Package:
24T/Box
Validity:
18 Months
Specimen:
Fish Fry Or Fish Heart, Intestine, Spleen, Kidney And Other Visceral Tissue Tissue Samples.
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
10 Copies/μL
Positive:
Ct≤38
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

PCR Fluorescent Strep Test Kit ISO

,

PCR Fluorescent Aquaculture Test Kit

,

Aquaculture Test Kit Iniae Septicaemia Virus

Product Description

PCR-Fluorescent probe kit Streptococcus iniae septicaemia virus Nucleic Acid Detection Kit

 
Intended use:

 

This kit is suitable for the specific detection of pathogenic Streptococcus iniae septicaemia virus in fish fry or viscera tissue samples such as heart, intestine, spleen and kidney.

 

Principle:
 

This kit designed primers with Streptococcus iniae septicaemia virus specific fragment to probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Streptococcus iniae septicaemia virus in the totally closed reaction system was realized by fluorescence PCR instrument.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples.

2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 94℃ 3min
2 45 94℃ 10s
60℃ 20s collect fluorescent

FAM was selected as the detection channel.

 

Quality control:

  1. Negative control: No obvious amplification curve of FAM detection channel;
  2. Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
  3. The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

The results of interpretation

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | > 0.980.

 

Product picture:

PCR Fluorescent Strep Test Kit Aquaculture Test Kit Iniae Septicaemia Virus 0

 

 

  Operation details please check from IFU!
 

products
PRODUCTS DETAILS
PCR Fluorescent Strep Test Kit Aquaculture Test Kit Iniae Septicaemia Virus
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W025
Package:
24T/Box
Validity:
18 Months
Specimen:
Fish Fry Or Fish Heart, Intestine, Spleen, Kidney And Other Visceral Tissue Tissue Samples.
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
10 Copies/μL
Positive:
Ct≤38
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

PCR Fluorescent Strep Test Kit ISO

,

PCR Fluorescent Aquaculture Test Kit

,

Aquaculture Test Kit Iniae Septicaemia Virus

Product Description

PCR-Fluorescent probe kit Streptococcus iniae septicaemia virus Nucleic Acid Detection Kit

 
Intended use:

 

This kit is suitable for the specific detection of pathogenic Streptococcus iniae septicaemia virus in fish fry or viscera tissue samples such as heart, intestine, spleen and kidney.

 

Principle:
 

This kit designed primers with Streptococcus iniae septicaemia virus specific fragment to probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Streptococcus iniae septicaemia virus in the totally closed reaction system was realized by fluorescence PCR instrument.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Fish fry or fish heart, intestine, spleen, kidney and other visceral tissue tissue samples.

2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 94℃ 3min
2 45 94℃ 10s
60℃ 20s collect fluorescent

FAM was selected as the detection channel.

 

Quality control:

  1. Negative control: No obvious amplification curve of FAM detection channel;
  2. Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
  3. The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

The results of interpretation

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | > 0.980.

 

Product picture:

PCR Fluorescent Strep Test Kit Aquaculture Test Kit Iniae Septicaemia Virus 0

 

 

  Operation details please check from IFU!
 

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