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Yellow Head Virus YHV Aquaculture Test Kit Pcr Test RT PCR

Yellow Head Virus YHV Aquaculture Test Kit Pcr Test RT PCR

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W011
Package:
24T/Box
Validity:
12 Months
Storage:
At -20±5℃, Protected From Light
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
10 Copies/μL
Linear:
| R | > 0.980
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

RT PCR Aquaculture Test Kit

,

YHV Aquaculture Test Kit

,

Yellow Head Virus Kit Pcr Test Biokey

Product Description

Yellow Head Virus (YHV) Nucleic Acid Detection Kit (RT-PCR-Fluorescent Probe Method)

 
Intended use:

 

This kit is suitable for the specific detection of pathogenic yellow head virus (YHV) in tissue samples such as gills, antennae glands, hematopoietic tissues and lymphatic organs of penaeus prawn.

 

Principle:
 

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat goblet virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of Prawn yellow head virus (YHV) was detected by fluorescence PCR in a completely closed reaction system.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Tissue samples from prawn

2. Sample collection

Samples were collected according to SNT 1151.4-2003.

Take fresh prawn tissue as sample.

2.1 For larvae or young shrimp, whole shrimp or shrimp heads are taken as samples. To remove the head, use sterilizing scissors and tweezers to remove the eyes, breastplate, all thoracic appendages, and abdomen behind the head.

2.2 For adult shrimp, gill filaments, lymphatic organs or hemolymph were taken as samples.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect the fluorescent

FAM is selected for detection channels.

 

Product performance index picture:

Yellow Head Virus YHV Aquaculture Test Kit Pcr Test  RT PCR 0

 

 

Quality control:

  1. Negative control: No obvious amplification curve of FAM detection channel;
  2. Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
  3. The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

The results of interpretation

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | > 0.980.

 

  Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Yellow Head Virus YHV Aquaculture Test Kit Pcr Test RT PCR
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W011
Package:
24T/Box
Validity:
12 Months
Storage:
At -20±5℃, Protected From Light
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
10 Copies/μL
Linear:
| R | > 0.980
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

RT PCR Aquaculture Test Kit

,

YHV Aquaculture Test Kit

,

Yellow Head Virus Kit Pcr Test Biokey

Product Description

Yellow Head Virus (YHV) Nucleic Acid Detection Kit (RT-PCR-Fluorescent Probe Method)

 
Intended use:

 

This kit is suitable for the specific detection of pathogenic yellow head virus (YHV) in tissue samples such as gills, antennae glands, hematopoietic tissues and lymphatic organs of penaeus prawn.

 

Principle:
 

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat goblet virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of Prawn yellow head virus (YHV) was detected by fluorescence PCR in a completely closed reaction system.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Tissue samples from prawn

2. Sample collection

Samples were collected according to SNT 1151.4-2003.

Take fresh prawn tissue as sample.

2.1 For larvae or young shrimp, whole shrimp or shrimp heads are taken as samples. To remove the head, use sterilizing scissors and tweezers to remove the eyes, breastplate, all thoracic appendages, and abdomen behind the head.

2.2 For adult shrimp, gill filaments, lymphatic organs or hemolymph were taken as samples.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect the fluorescent

FAM is selected for detection channels.

 

Product performance index picture:

Yellow Head Virus YHV Aquaculture Test Kit Pcr Test  RT PCR 0

 

 

Quality control:

  1. Negative control: No obvious amplification curve of FAM detection channel;
  2. Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
  3. The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

The results of interpretation

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | > 0.980.

 

  Operation details please check from IFU!
 

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