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Diagnostic Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit

Diagnostic Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W018
Package:
24T/Box
Validity:
12 Months
Specimen:
Samples Of Animal Tissue, Feed, Feces, Etc
Applicable:
Micgene 242 / 244 / 244IVD
Mini Limit:
10 Copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Diagnostic Multiplex Test Pcr scrv

,

Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus

,

Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit

Product Description

Diagnostic / Laboratory virus Fluorescence PCR kit Siniperca chuatsi rhabdovirus Nucleic Acid PCR Kit

 
Intended use:

 

This kit is suitable for auxiliary diagnosis of Siniperca chuatsi rhabdovirus (SCRV) infection in animal tissue, feed, feces and other samples.

 

Principle:
 

This kit to choose a conservative design specific gene probe, the probe can with primer amplification area in the middle of Siniperca chuatsi rhabdovirus (SCRV) specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Siniperca chuatsi rhabdovirus (SCRV) nucleic acid in the completely closed reaction system was realized by fluorescence PCR instrument.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Samples of animal tissue, feed, feces, etc

2. Sample collection and transportation

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. In which, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimps.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 50℃ 5min
2 1 95℃ 5min
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM was selected for detection channel

 

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: The detection limit of this kit is 10 copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

 

Quality control:

 

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.


  Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Diagnostic Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W018
Package:
24T/Box
Validity:
12 Months
Specimen:
Samples Of Animal Tissue, Feed, Feces, Etc
Applicable:
Micgene 242 / 244 / 244IVD
Mini Limit:
10 Copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Diagnostic Multiplex Test Pcr scrv

,

Multiplex Test Pcr Siniperca Chuatsi Rhabdovirus

,

Siniperca Chuatsi Rhabdovirus Nucleic Acid Test Kit

Product Description

Diagnostic / Laboratory virus Fluorescence PCR kit Siniperca chuatsi rhabdovirus Nucleic Acid PCR Kit

 
Intended use:

 

This kit is suitable for auxiliary diagnosis of Siniperca chuatsi rhabdovirus (SCRV) infection in animal tissue, feed, feces and other samples.

 

Principle:
 

This kit to choose a conservative design specific gene probe, the probe can with primer amplification area in the middle of Siniperca chuatsi rhabdovirus (SCRV) specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Siniperca chuatsi rhabdovirus (SCRV) nucleic acid in the completely closed reaction system was realized by fluorescence PCR instrument.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Samples of animal tissue, feed, feces, etc

2. Sample collection and transportation

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. In which, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimps.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 50℃ 5min
2 1 95℃ 5min
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM was selected for detection channel

 

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: The detection limit of this kit is 10 copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

 

Quality control:

 

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.


  Operation details please check from IFU!
 

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