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DNA Taq Aquaculture Test Kit Grass Carp Reovirus Virus GCRVI Type I PCR Test Kit

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DNA Taq Aquaculture Test Kit Grass Carp Reovirus Virus GCRVI Type I PCR Test Kit

DNA Taq Aquaculture Test Kit Grass Carp Reovirus  Virus GCRVI Type I PCR Test Kit
DNA Taq Aquaculture Test Kit Grass Carp Reovirus  Virus GCRVI Type I PCR Test Kit DNA Taq Aquaculture Test Kit Grass Carp Reovirus  Virus GCRVI Type I PCR Test Kit

Large Image :  DNA Taq Aquaculture Test Kit Grass Carp Reovirus Virus GCRVI Type I PCR Test Kit

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: ISO 13485
Model Number: BIK-Q-W022
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day

DNA Taq Aquaculture Test Kit Grass Carp Reovirus Virus GCRVI Type I PCR Test Kit

Description
Package: 24T/Box Validity: 12 Months
Storage: At -20±5℃, Protected From Light For 18 Months Applicable: Micgene 242 / 244 、ABI QuantStudio5
Mini Limit: 3 Copies/μL CV: ≤5%
High Light:

ISO DNA Taq Aquaculture Test Kit

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GCRVI Aquaculture Test Kit

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Grass Carp Reovirus Virus PCR Test Kit

DNA Taq polymeraset detect Grass Carp Reovirus virus ( GCRVI ) Type I PCR test kit

 
Intended use:

 

This kit is suitable for auxiliary diagnosis of Grass Carp Reovirus Type I virus (GCRVI) infection

 

Principle:
 

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose peach virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of taora virus was detected by fluorescence PCR in a completely closed reaction system.​
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:
 

1. Sample type

Tissue samples of grass carp and black carp

2. Sample collection

Fry with body length ≤4cm should be taken as a whole fish, if there is yolk sac, it should be removed; All viscera including kidney and brain were taken from 4~6cm fry. Liver, brain, spleen and kidney were taken from fish with body length ≥6cm. 5 fish can be combined into a small sample.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.
 
The results of interpretation:
 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 
Product performance index:
 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 3copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.


  Operation details please check from IFU!
 

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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