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Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit

Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W026
Package:
24T/Box
Validity:
18 Months
Storage:
At -20±5℃, Protected From Light For 18 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
10 Copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

CCV Channel Catfish Virus Rt Pcr Kit

,

CCV Rt Pcr Kit Test Septicaemia Virus

,

Septicaemia Virus QPCR Kit

Product Description

Channel Catfish Virus Septicaemia Virus ( CCV ) Nucleic Acid DNA Amplification qPCR Kit

 
Intended use:
 
This kit is suitable for Channel catfish virus septicaemia virus (CCV) of liver, spleen and kidney tissue samples.

 

Principle:
 

This kit selection Channel catfish virus septicaemia virus (CCV) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Fluorescence PCR instrument was used to detect Channel catfish virus septicaemia virus CCV in a completely closed reaction system.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
Aquatic CCV Channel Catfish Virus RT PCR  Test Septicaemia Virus  Kidney tissue qPCR Kit 0
  
Sample requirements:
 

1. Sample type
Channel catfish”Liver, spleen, kidney, etc

2.Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s fluorescent

FAM is selected for detection channels.
 
The results of interpretation:
 

Results Ct Value
Positive Ct≤38
Negative

No Ct value or Ct value≥45

Gray area 38<Ct≤45

 
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 
Product performance index:
 
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
 
 
 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Aquatic CCV Channel Catfish Virus RT PCR Test Septicaemia Virus Kidney tissue qPCR Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W026
Package:
24T/Box
Validity:
18 Months
Storage:
At -20±5℃, Protected From Light For 18 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
10 Copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

CCV Channel Catfish Virus Rt Pcr Kit

,

CCV Rt Pcr Kit Test Septicaemia Virus

,

Septicaemia Virus QPCR Kit

Product Description

Channel Catfish Virus Septicaemia Virus ( CCV ) Nucleic Acid DNA Amplification qPCR Kit

 
Intended use:
 
This kit is suitable for Channel catfish virus septicaemia virus (CCV) of liver, spleen and kidney tissue samples.

 

Principle:
 

This kit selection Channel catfish virus septicaemia virus (CCV) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Fluorescence PCR instrument was used to detect Channel catfish virus septicaemia virus CCV in a completely closed reaction system.
 
Main components:
 

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
Aquatic CCV Channel Catfish Virus RT PCR  Test Septicaemia Virus  Kidney tissue qPCR Kit 0
  
Sample requirements:
 

1. Sample type
Channel catfish”Liver, spleen, kidney, etc

2.Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s fluorescent

FAM is selected for detection channels.
 
The results of interpretation:
 

Results Ct Value
Positive Ct≤38
Negative

No Ct value or Ct value≥45

Gray area 38<Ct≤45

 
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 
Product performance index:
 
1. Accuracy
The positive reference materials of testing enterprises were all positive.
2. Minimum detection limit: the detection limit of this kit is 10copies/μL.
3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.
4. Linear, linear correlation coefficient | r | > 0.980.
 
 
 Operation details please check from IFU!
 

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