|Storage:||At -20±5℃, Protected From Light For 12 Months||Applicable:||Micgene 242 / 244 、ABI QuantStudio5|
SHIV Aquaculture Test Kit,
Biokey Aquaculture Test Kit Nucleic Acid QPCR,
SHIV QPCR Fluorescent Quantitative Test Kit
Shrimp Hemocyte Iridescent Virus (SHIV) Nucleic Acid Detection Kit (PCR-Fluorescent Probe Method)
This kit is suitable for the detection of Shrimp Hemocyte Iridescent Virus (SHIV) infection in adult prawn, prawn parent, prawn seedling, bait, processed aquatic products, etc.
This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.
|PCR reaction liquid||24 tubes|
|Positive control||1 tube|
|Negative control||1 tube|
1. Sample type
Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
|60℃||30s collect fluorescent|
FAM is selected for detection channels.
The results of interpretation:
|Negative||No Ct value or Ct value≥45|
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Contact Person: Lisa