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Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit

Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W008
Package:
24T/Box
Validity:
18 Months
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
Highlight:

SHIV Aquaculture Test Kit

,

ISO Aquaculture Test Kit Shrimp Hemocyte Iridescent

,

SHIV PCR Detection Kit

Product Description

Shrimp Hemocyte Iridescent Virus ( SHIV ) Nucleic Acid Primer Probe PCR Detection Kit


 
Intended use:

 

This kit is suitable for the detection of Shrimp Hemocyte Iridescent Virus (SHIV) infection in adult prawn, prawn parent, prawn seedling, bait, processed aquatic products, etc.

 

Principle:

 

This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Shrimp Hemocyte Iridescent Virus Aquaculture Test Kit SHIV PCR Detection Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W008
Package:
24T/Box
Validity:
18 Months
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
Highlight

SHIV Aquaculture Test Kit

,

ISO Aquaculture Test Kit Shrimp Hemocyte Iridescent

,

SHIV PCR Detection Kit

Product Description

Shrimp Hemocyte Iridescent Virus ( SHIV ) Nucleic Acid Primer Probe PCR Detection Kit


 
Intended use:

 

This kit is suitable for the detection of Shrimp Hemocyte Iridescent Virus (SHIV) infection in adult prawn, prawn parent, prawn seedling, bait, processed aquatic products, etc.

 

Principle:

 

This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

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