|Storage:||Store At Room Temperature, Away From Light||Applicable:||Micgene 242 / 244 、ABI QuantStudio5|
Enterocytozoon Hepatopenaei Pcr Diagnostic Kit,
Pcr Diagnostic Kit Acute Hepatopancreatic Necrosis,
Enterocytozoon Hepatopenaei qpcr test kit
Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit
This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.
This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.
|PCR reaction liquid||24 tubes|
|Positive control||1 tube|
|Negative control||1 tube|
1. Sample type
Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples
2. Sample collection and transportation
Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Using Micgene 242/244/244IVD and ABI QuantStudio5:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
|60℃||30s collect fluorescent|
FAM and VIC/HEX are selected for detection channels.
|FAM channel (EMS)||VIC/HEX channel(EHP-18S)||Results judgment|
|1||-||-||The results were reported negative|
|2||+||-||The results were reported EMS positive|
|3||-||+||The results were reported EHP-18S positive|
|4||+||+||The results were reported EMS /EHP-18S positive|
Note: "+" means positive, "-" means negative
The results of interpretation
|Negative||No Ct value or Ct value=0|
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as
Operation details please check from IFU!
Contact Person: Lisa