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Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

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Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit
Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Large Image :  Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: ISO 13485
Model Number: BIK-Q-W015
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day

Shrimp Enterocytozoon Hepatopenaei Acute Hepatopancreatic Necrosis PCR Diagnostic kit

Description
Package: 24T/Box Validity: 18 Months
Storage: Store At Room Temperature, Away From Light Applicable: Micgene 242 / 244 、ABI QuantStudio5
LoD: 10copies/μL CV: ≤5%
High Light:

Enterocytozoon Hepatopenaei Pcr Diagnostic Kit

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Pcr Diagnostic Kit Acute Hepatopancreatic Necrosis

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Enterocytozoon Hepatopenaei qpcr test kit

Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit


 
Intended use:

 

This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.


Principle:

 

This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.


Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples

 

2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

 

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 40 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

Result interpretation:

 

  FAM channel (EMS) VIC/HEX channel(EHP-18S) Results judgment
1 - - The results were reported negative
2 + - The results were reported EMS positive
3 - + The results were reported EHP-18S positive
4 + + The results were reported EMS /EHP-18S positive

Note: "+" means positive, "-" means negative

 

The results of interpretation

 

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤40

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as


 Operation details please check from IFU!
 

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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