Send Message
products
PRODUCTS DETAILS
Home > Products >
Taura Syndrome Virus Aquaculture Test Kit Fluorescent Probe PCR Amplification

Taura Syndrome Virus Aquaculture Test Kit Fluorescent Probe PCR Amplification

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W012
Package:
24T/Box
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Taura Syndrome Virus Aquaculture Test Kit

,

Aquaculture Test Kit Fluorescent Probe

,

TSV Fluorescent Probe PCR Aquaculture Kit

Product Description

Taura syndrome virus (TSV)Nucleic Acid Detection Kit 

(PCR-Fluorescent Probe Method)


 
Intended use:
This kit is suitable for auxiliary diagnosis of Taura syndrome virus infection.


Principle:


This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose peach virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of taora virus was detected by fluorescence PCR in a completely closed reaction system.

Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Tissue samples from prawn

2. Sample collection

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. In which, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimps.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 
procedures are set as below:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 

Result analysis:
Automatic analysis using instrumentation
 
Quality control:
Negative control: No obvious amplification curve of FAM detection channel;
Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
 
The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

 
Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Taura Syndrome Virus Aquaculture Test Kit Fluorescent Probe PCR Amplification
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W012
Package:
24T/Box
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
LoD:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Taura Syndrome Virus Aquaculture Test Kit

,

Aquaculture Test Kit Fluorescent Probe

,

TSV Fluorescent Probe PCR Aquaculture Kit

Product Description

Taura syndrome virus (TSV)Nucleic Acid Detection Kit 

(PCR-Fluorescent Probe Method)


 
Intended use:
This kit is suitable for auxiliary diagnosis of Taura syndrome virus infection.


Principle:


This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose peach virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of taora virus was detected by fluorescence PCR in a completely closed reaction system.

Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Tissue samples from prawn

2. Sample collection

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. In which, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimps.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 
procedures are set as below:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 

Result analysis:
Automatic analysis using instrumentation
 
Quality control:
Negative control: No obvious amplification curve of FAM detection channel;
Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
 
The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

 
Operation details please check from IFU!
 

Sitemap |  Privacy Policy | China Good Quality RT qPCR Machine Supplier. Copyright © 2022-2024 Guangzhou BioKey Healthy Technology Co.Ltd . All Rights Reserved.