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Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR Kit Quarantine

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China Guangzhou BioKey Healthy Technology Co.Ltd certification
China Guangzhou BioKey Healthy Technology Co.Ltd certification
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Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR Kit Quarantine

Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR  Kit Quarantine
Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR  Kit Quarantine

Large Image :  Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR Kit Quarantine

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: ISO 13485
Model Number: BIK-Q-W004
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day

Enterocytozoon Hepatopenaei Aquaculture Test Kit EHP Taqman QPCR Kit Quarantine

Description
Package: 24T/Box Validity: 12 Months
Storage: Store Away From Direct Light At -20±5℃ Applicable: Micgene 242 / 244 、ABI QuantStudio5
Limit Of Detection: 3copies/μL CV: ≤5%
High Light:

Enterocytozoon Hepatopenaei Aquaculture Test Kit

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Aquaculture Test Kit EHP

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ISO EHP Taqman QPCR Kit Quarantine

Enterocytozoon hepatopenaei Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)
 
 
Intended use:
 
This kit is applied for  quarantine inspection 
of dectecting Enterocytozoon hepatopenaei(EHP) virus before buying larvae in shrimp desalt plant to ensure that larvae do not carry the disease pathogen. Aquaculture enterprises have conducted EHP specific detection on the bottom mud of aquaculture ponds with slow growth of prawn to exclude the hidden danger of liver and intestinal cytiworm parasitization.
 
Principle:
 
This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system.
 
Main components:
 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 
Sample requirements:
 

1. Sample type

Seedlings: parent, shrimp eggs, seedlings, coarse standard products

Pond: bottom mud, excrement, water body

Bait: live bait, chilled bait and non-high-temperature treated bait samples

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.
 
Using Micgene 242/244/244IVD and ABI QuantStudio5:
 
procedures are set as below:

Steps

Cycle number

Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃

30sCollect the fluorescent

FAM was selected for detection channel.
 
The results of interpretation

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value≥45
Gray area 40<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
 
Result analysis:
Automatic analysis using instrumentation
 
Quality control:
Negative control: No obvious amplification curve of FAM detection channel;
Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;
 
The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.
 

Details please check from IFU!
 

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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