|Application:||Gene & Life Science Lab Consumables||Warranty:||1 Year|
|Function:||Nucleic Acid Extraction||Specimen:||Serum, Plasma,nasal/pharyngeal Swab,etc.|
Magnetic Bead Automated Rna Extraction Kit,
Viral Nucleic Acid Extraction Kit 1.1mL,
Magnetic Bead Viral Nucleic Acid Extraction Kit
General purpose magnetic bead 48 samples virus DNA / RNA
rapid extraction kit
Intend for Use:
This product does not need sample pretreatment, and the same reagent can simultaneously meet the extraction and purification of DNA virus and RNA virus from serum, plasma and culture medium of cotton swab. The processed products are used for scientific research and testing.
|Preloaded deep hole plate||1 test/strip, 3 boards/box|
|Protease K||1.1mL/ tube, 1 tube/box|
|Magnetic sleeve rod||
12 magnetic rod sleeve
Storage Conditions and Expiry date:
Dry at room temperature (20 ~ 25℃), this product is valid for 12 months. Please refer to the product packaging label for the production batch number.
1. Sample type: 200 μL of human whole blood or acellular body fluid (such as serum, plasma, cerebrospinal fluid, nasal/pharyngeal swab, alveolar lavage fluid, etc.)
2. Specimen Collection:
2.1 Anticoagulant whole blood: 2ml of venous blood was drawn with a disposable sterile syringe and injected into the anticoagulant tube EDTA or sodium citrate. Immediately, the glass tube was gently reversed and mixed for 5 to 10 times, so that the anticoagulant and venous blood were fully mixed.
2.2 Serum: 2ml of venous blood was drawn with a disposable sterile syringe and injected into a sterile dry glass tube. When the blood samples were placed at room temperature (15-30 ℃) for 30-60 minutes or 4 ℃ for 2 hours, the serum could be completely agglutinated spontaneously, or centrifuged at 1500 RPM for 5 minutes directly using a horizontal centrifuge. The upper serum was absorbed and transferred to a 1.5ml centrifuge tube for use.
2.3 Plasma: 2ml of venous blood was drawn with a disposable sterile syringe and injected into an EDTA or sodium citrate anticoagulant tube. Immediately, the glass tube was gently reversed and mixed for 5 to 10 times, so that the anticoagulant and venous blood were fully mixed. After 5-10 minutes, the upper plasma could be separated and transferred to a 1.5 mL centrifuge tube for reserve.
2.4 CEREBROspinal fluid (CSF) : CSF is collected by lumbar puncture, and can be obtained from cerebellar medulla cisterna or lateral ventricle if necessary. Pressure measurement should be performed by a physician after puncture. Cerebrospinal fluid pressure can increase when any lesion increases the volume of brain tissue or cerebrospinal fluid. Cerebrospinal fluid was collected in sterile test tubes after pressure measurement.
2.5 Pharyngeal swab: Swab both pharyngeal tonsils and posterior pharyngeal wall with two plastic rods with polypropylene fiber heads. Dip the swab head into a tube containing 3ml sampling solution, discard the tail, and tighten the tube cap. For highly pathogenic respiratory tract infections, it is recommended to inactivate them with water bath at 56 ℃ for 30min.
2.6 Nasal swab: Gently insert a plastic rod with a polypropylene fiber head into the nasal canal at the nose and palate, stay for a while, and then slowly rotate out. A plastic rod swab from another polypropylene fiber head was taken from the other nostril in the same manner. Dip the two swabs into the same tube containing 3ml sample solution, discard the tail and tighten the tube cap. For highly pathogenic respiratory tract infections, water bath at 56 ℃ for 30min is recommended for inactivation.
2.7 Alveolar irrigation fluid: after local anesthesia, insert the bronchoscope through the mouth or nose through the pharynx into the branch of the middle lobe of the right lung or the tongue segment of the left lung, insert the top of the bronchial branch into the opening, slowly add sterilized normal saline through the trachea biopsy hole, 30~50 ml each time, the total amount is 100~250 ml, should not exceed 300 mL. For highly pathogenic respiratory tract infections, it is recommended to inactivate them with water bath at 56 ℃ for 30min.
3. Specimen storage and transportation: Specimens can be immediately used for testing, or stored in -70 ℃ or lower temperature for testing, storage period of 6 months, should avoid repeated freezing and thawing. Specimens are transported by cold chain.
4. Freezing and thawing requirements: quick freezing and thawing, avoid repeated freezing and thawing.
1 Take out the reagent strip adaptor (K12), and insert the reagent strip into the adaptor (the a-end of the reagent strip (the Angle missing end) is in the same direction as the A-end of the adaptor).
2 Add 200 µ L sample and 20μ L protease K to the first well at the A end of the reagent strip.
3 Put the adapter into the base clip of the nucleic acid extraction instrument, insert 12 magnetic rod sleeve into the clip slot of the magnetic rod sleeve, open file management on the touch screen, select "BMVF-K" program, and click Load - Run. Note: Place the adapter in the base clamp with "H" on the left and "A" on the right. If it is incorrectly placed, virus nucleic acid cannot be obtained.
4 The extracted viral nucleic acid is stored in the "H" row. If it is not used immediately, please transfer it to a 1.5ml sterile nuclease free centrifuge tube and store it at -15~-25 ℃ or lower temperature. Please store it at -70 ℃ for long-term storage.
Contact Person: Ms. Lisa