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Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe

Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe

MOQ: we produce both liquid and lyophilized kit
Price: USD
Standard Packaging: Carton Package
Delivery Period: depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO13485
Model Number
Bik-Q-P039
Specimen:
Feline/dog Droppings.
Packing:
8T/box
Linear:
| R | >0.980
LOD:
10 Copies/μL
CV:
≤5%
Applicable:
Micgene 242/244/244 IVD
Minimum Order Quantity:
we produce both liquid and lyophilized kit
Price:
USD
Packaging Details:
Carton Package
Delivery Time:
depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Dog Helicobacter Test Kit

,

Helicobacter Test Kit PCR

,

Canine h pylori DNA Detection Kit

Product Description

Canine / Dog Helicobacter pylori ( Hp ) Bacteria DNA Detection Kit ( PCR-Fluorescent Probe )                    


Intend for Use:
 
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.
 
Principle:
 
This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.
 
Component:

 

Composition Quantity
PCR Liquid 8 tube
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

Sample requirements:

 

1. Sample type

Cat/dog droppings.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 94℃ 3min
2 45 94℃ 10s
60℃ 20s collection fluorescent

FAM was selected for detection channel

 

 

Positive judgment value or reference interval:

 

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | >0.980.

 

Product picture:

Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe 0

products
PRODUCTS DETAILS
Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe
MOQ: we produce both liquid and lyophilized kit
Price: USD
Standard Packaging: Carton Package
Delivery Period: depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO13485
Model Number
Bik-Q-P039
Specimen:
Feline/dog Droppings.
Packing:
8T/box
Linear:
| R | >0.980
LOD:
10 Copies/μL
CV:
≤5%
Applicable:
Micgene 242/244/244 IVD
Minimum Order Quantity:
we produce both liquid and lyophilized kit
Price:
USD
Packaging Details:
Carton Package
Delivery Time:
depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Dog Helicobacter Test Kit

,

Helicobacter Test Kit PCR

,

Canine h pylori DNA Detection Kit

Product Description

Canine / Dog Helicobacter pylori ( Hp ) Bacteria DNA Detection Kit ( PCR-Fluorescent Probe )                    


Intend for Use:
 
This kit is suitable for the specific detection of pathogenic Helicobacter pylori (Hp) in cat/dog feces samples.
 
Principle:
 
This kit selection of helicobacter pylori (Hp) specific fragment to design primers probe probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to achieve the detection of Helicobacter pylori nucleic acid (Hp) in the completely closed reaction system.
 
Component:

 

Composition Quantity
PCR Liquid 8 tube
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

Sample requirements:

 

1. Sample type

Cat/dog droppings.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

PCR amplification and fluorescence detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 94℃ 3min
2 45 94℃ 10s
60℃ 20s collection fluorescent

FAM was selected for detection channel

 

 

Positive judgment value or reference interval:

 

Results Ct Value
Positive Ct≤38
Negative No Ct value or Ct value≥45
Gray area 38<Ct<45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 10copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

4. Linear, linear correlation coefficient | r | >0.980.

 

Product picture:

Dog Helicobacter Test Kit Bacteria DNA Detection Kit PCR Fluorescent Probe 0

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