|Package:||8T/Box||Shelf Life:||12 Months|
|Storage:||-20±5℃||Applicable:||Micgene 242/244/244 PCR|
|LoD:||3 Copies / μL||CV:||≤5%|
Canine Babesiosis Pcr Kit,
Babesiosis Pcr Kit EDTA,
Babesiosis Nucleic Acid Testing Kit
Canine Babesiosis (B.C) Nucleic acid testing kit
(PCR-Fluorescent Probe Method)
This kit is suitable for the specific detection of Babesiosis (B.C) in canine EDTA whole blood and other samples.
This kit uses the conserved gene fragment of Canine Babesiosis (B.C) to design a specific probe. The probe can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of the Taq enzyme will 5 The '-end fluorescent group is cleaved from the probe, making it free in the reaction system, thereby detaching from the 3'-end quenching group and emitting fluorescence, which is detected by a fluorescence PCR instrument to realize the detection of Canine Babesiosis (B.C) in a fully closed reaction system. Detection of Beth worm nucleic acid.
|PCR reaction liquid||8 tubes|
|Positive control||1 tube|
|Negative control||1 tube|
1. Sample type
Canine EDTA whole blood etc.
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
|60℃||30s collect fluorescent|
FAM is selected for detection channels.
Positive judgment value or reference interval
|Negative||No Ct value or Ct value≥45|
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Contact Person: Lisa