SARS-CoV-2 / Influenza A+B RT-PCR test kit (Lyophilized)
This kit is used for in vitro qualitative detection of SARS-CoV-2/Influenza A+B from patients with respiratory tract
infection. The test results are for clinical reference only, it cannot be used alone as the basis for the diagnosis or
exclusion of cases
The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of SARS-CoV-2/Influenza A+B,
the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different
fluorescent groups. On the fluorescence quantitative PCRinstrument, the real-time fluorescence quantitative PCR
detec tion technology is used to realize the rapid detection of SARS-CoV-2/Influenza A+B through the change of fluorescent
signal.The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.
||PCR Enzyme Mix (Lyophilized)
||Enzyme Mix Buffer (5×)
Note: Do not mix the components from different batches for
detection. The Positive Control of SARS-CoV-2/Influenza
A+B and internal control were constructed artificially, and
they were not infectious.
Storage and stability:
1.The kit should be stored under -20±5℃ and protected from light. Its period of validity is 12 months. Products
can be shipped at room temperature for 7 days.
2.Repeated thawing and freezing should be no more than 3 times
1. Specimen type
Throat swab, nasal swab, nasopharyngeal swab, alveolar lavage fluid and sputum.
2. Specimen preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃.
PCR amplification condition:
PCR amplification according to the following procedure:
||30s Collect the fluorescent
Select the detection channels as following:
Select FAM,VIC/HEX,ROX and CY5 channels
Use national reference products or enterprise positive/negative
reference products for testing, and the results are all
The coefficient of variation (CV) of CT value is ≤5.0%.
3. Detection limit: 500 copies/mL.
Operation details please check from IFU