Multiplex Respiratory Syncytial Virus RSV Streptococcus Pneumoniae Adenovirus PCR Test

Name: Multiplex Respiratory Syncytial Virus RSV Streptococcus Pneumoniae Adenovirus PCR Test
Brand: Biokey
SKU: BIK-QL-H004 / BIK-QL-H004S
Price: 1.00 USD
Availability: InStock
Rating: 4.7 (163 reviews)

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Respiratory Syncytial Virus / Influenza B / A RT PCR Test Kit Lyophilized 48tests/Kit

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Multiplex Respiratory Syncytial Virus RSV Streptococcus Pneumoniae Adenovirus PCR Test

Large Image : Multiplex Respiratory Syncytial Virus RSV Streptococcus Pneumoniae Adenovirus PCR Test

Product Details:

Place of Origin:	Guangzhou China
Brand Name:	Biokey
Certification:	ISO 13485,CE
Model Number:	BIK-QL-H004 / BIK-QL-H004S

Payment & Shipping Terms:

Minimum Order Quantity:	We can produce liquid and lyophilized kits
Price:	USD
Packaging Details:	Carton package
Delivery Time:	Depending on order quantity
Payment Terms:	L/C, T/T, Western Union
Supply Ability:	100,000 per day

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Multiplex Respiratory Syncytial Virus RSV Streptococcus Pneumoniae Adenovirus PCR Test

Description

Package:	48 T/kit(single Pcr Tube)	Specimen Type:	Throat Swab, Nasal Swab, Nasopharyngeal Swab, Alveolar Lavage Fluid And Sputum.
Specimen Preservation:	Stored For 3 Months At -20±5℃ And For A Long Time Below -70℃	Applicable:	Micgene 244/244 IVD(BIO-KEY)
LoD:	500 Copies/mL	CV:	≤5%
High Light:	RSV M Pneumoniae PCR Test Kit , Multiplex M Pneumoniae PCR Test Kit , Kit Adenovirus Pcr Test Multiplex

Multiplex Respiratory viral RT-PCR test kit Lyophilized

Intended use:

This kit is used for in vitro qualitative detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae from patients with respiratory tract infection. The test results are for clinical reference only, it cannot be used alone as the basis for the diagnosis or exclusion of cases

Test Principle:

The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae, the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different fluorescent groups. On the fluorescence quantitative PCR instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae through the change of fluorescent signal.The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.The kit adopt TaqMan fluorescent probe method. For the highly conserved and specific region of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae, the primer probes, together with RT-PCR reaction solutionfor fluorescent detection are designed and labeled with different fluorescent groups. On the fluorescence quantitative PCR instrument, the real-time fluorescence quantitative PCR detection technology is used to realize the rapid detection of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae through the change of fluorescent signal.The detection system contains positive internal control, used to monitor whether the nucleic acid extraction process is normal.

Biokey Multiple Respiratory PCR testing solution.p...

Product components:

1. BIK-QL-H004:

No.	Components	Amount
1	PCR Enzyme Mix (Lyophilized)	96 tests/bottle
2	Primer-Probe Mix 1 (SARS-CoV-2/Influenza A+B )	50 μL/vial
3	Primer-Probe Mix 2 (RSV/Adenovirus/M.pneumoniae)	50 μL/vial
4	Enzyme Mix Buffer (5×)	400 μL/vial
5	Positive Control	100 μL/tube
6	Negative Control	100 μL/tube
7	RNase-free water	1mL/tube
8	Paraffin oil	1.5mL/tube*2

2. BIK-QL-H004S:

No.	Components	Amount
1	1 (SARS-CoV-2/Influenza A+B Mix (Lyophilized))	24 tests/kit
2	2 (RSV/Adenovirus/M.pneumoniae Mix (Lyophilized))	24 tests/kit
3	Positive Control	100 μL/tube
4	Negative Control	100 μL/tube
5	RNase-free water	1mL/tube
6	Paraffin oil	1.5mL/tube

Note: Do not mix the components from different batches for detection. The Positive Control of SARS-CoV-2/Influenza A+B/RSV/Adenovirus/M.pneumoniae and internal control were constructed artificially, and they were not infectious.

Storage and stability:

1.The kit should be stored under -20±5℃ and protected from light. Its period of validity is 12 months. Products can be shipped at room temperature for 7 days.

2.Repeated thawing and freezing should be no more than 3 times.

PCR amplification condition:

PCR amplification according to the following procedure:

Step	Cycle number	Temperature	Time
1	1	50℃	10min
2	1	94℃	3min
3	40	94℃	10s
3	40	60℃	30s Collect the fluorescent

Select the detection channels as following:

Select FAM,VIC/HEX,ROX and CY5 channels

Quality control:

Negative Control: None of the FAM, VIC/HEX, ROX & IC (CY5) channels have a Ct value or Ct ＞ 40;

Positive Control: FAM, VIC/HEX, ROX & IC (CY5) channels Ct ≤ 30;

The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.

Interpretation of test results:

RSV/Adenovirus/M.pneumoniae reaction:

	FAM (RSV)	VIC/HEX (Adenovirus)	ROX (M.pneumonia)	CY5 (IC)	Interpretation test results
1	-	-	-	-	Result invalid ,test again
2	+	-	-	+/-	RSV(+)
3	-	+	-	+/-	Adenovirus(+)
4	-	-	+	+/-	M.pneumonia(+)
5	+	+	-	+/-	RSV,Adenovirus(+)
6	+	-	+	+/-	RSV,M.pneumonia(+)
7	-	+	+	+/-	Adenovirus, M.pneumonia (+)
8	+	+	+	+/-	RSV, Adenovirus, M.pneumonia(+)
9	-	-	-	+	Negative(-)

Operation details please check from IFU

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