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Bb Feline Cat Test Kit Bordetella Bronchiseptica RT QPCR Taqman Probe Detection Kit

Bb Feline Cat Test Kit Bordetella Bronchiseptica RT QPCR Taqman Probe Detection Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P023
Package:
8T/Box
Shelf Life:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies / μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Bordetella Bronchiseptica Feline Cat Test Kit

,

Bordetella Bronchiseptica RT QPCR Test Kit

,

Bb QPCR Test Kit Taq

Product Description

Cat Bordetella bronchiceptica(Bb) RT qPCR test kit , Taq DNA polymerase probe testing kit

 


Intended use:

 

This kit is suitable for the specific detection of Bordetella bronchiceptica (Bb) in eye, mouth and nose secretions.


Principle:

 

This kit to choose Bordetella bronchiceptica (Bb) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Bordetella bronchiceptica (Bb) nucleic acid in a completely closed reaction system was realized by fluorescence PCR instrument.

 

Biokey Pet PCR testing solution.pdf

 

Main components:

 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Eye, mouth and nose secretions.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

Positive judgment value or reference interval

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value≥45
Gray area 40<Ct<45

 

 

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Bb Feline Cat Test Kit Bordetella Bronchiseptica RT QPCR Taqman Probe Detection Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P023
Package:
8T/Box
Shelf Life:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies / μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Bordetella Bronchiseptica Feline Cat Test Kit

,

Bordetella Bronchiseptica RT QPCR Test Kit

,

Bb QPCR Test Kit Taq

Product Description

Cat Bordetella bronchiceptica(Bb) RT qPCR test kit , Taq DNA polymerase probe testing kit

 


Intended use:

 

This kit is suitable for the specific detection of Bordetella bronchiceptica (Bb) in eye, mouth and nose secretions.


Principle:

 

This kit to choose Bordetella bronchiceptica (Bb) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The detection of Bordetella bronchiceptica (Bb) nucleic acid in a completely closed reaction system was realized by fluorescence PCR instrument.

 

Biokey Pet PCR testing solution.pdf

 

Main components:

 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Eye, mouth and nose secretions.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

Positive judgment value or reference interval

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value≥45
Gray area 40<Ct<45

 

 

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

 Operation details please check from IFU!
 

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