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Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq

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Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq

Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq
Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq

Large Image :  Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: ISO 13485
Model Number: BIK-Q-P035
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day

Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq

Description
Package: 8T/Box Validity: 12 Months
Storage: -20±5℃ Applicable: Micgene 242/244/244 IVD PCR
LoD: 10copies/μL CV: ≤5%
High Light:

Leukemia Feline Cat Test Kit QPCR

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Feline Cat Test Kit QPCR AIDS

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FIV FeLV Test Kit DNA Taq

Veterinary Feline leukemia virus/Feline AIDS virus (FeLV/FIV) DNA taq polymerase probe qPCR kit

 

 

Intended use:

 

This kit is suitable for the specific detection of feline leukemia virus (FeLV) and feline AIDS virus (FIV) in EDTA whole blood sample.


Principle:

 

Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose feline leukemia virus (FeLV) and feline AIDS virus (FIV) gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to realize nucleic acid detection of f feline leukemia virus (FeLV) and feline AIDS virus (FIV) virus in the completely closed reaction system.


Main components:
 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 
Product pictures:
 
Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq 0
Veterinary Leukemia Feline Cat Test Kit QPCR AIDS FIV FeLV Test Kit DNA Taq 1

 
Sample requirements:
 

1. Sample type

EDTA whole blood

 

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

 

3. Sample preservation

 It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
 
PCR amplification and fluorescence Detection (PCR detection area):

 

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

The results of interpretation:

 

  FAM channel (FeLV) VIC/HEX channel (FIV) Result interpretation
1 - - Results were repoFIVrted negative
2 + - Results were reported FeL positive
3 - + Results were reported FIV positive
4 + + Results were reported FeLV,FIV positive

Note: "+" means positive, "-" means negative

 

Positive judgment value or reference interval:

 

Result Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 

 Operation details please check from IFU!
 

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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