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Real Time Feline Herpes Test Kit QPCR Feline Calicivirus Test Kit

Real Time Feline Herpes Test Kit QPCR Feline Calicivirus Test Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P030
Package:
8T/Box
Validity:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

QPCR Feline Calicivirus Test Kit

,

Feline Herpes Test Kit QPCR

,

Real Time feline calicivirus pcr

Product Description

Feline Herpes virus/Feline Caliciviral virus Detection kit

(Real time PCR probe Method)

 
Intended use:

 

This kit is suitable for the specific detection of feline herpes virus (FHV) and feline calicivirus (FCV) in cat eye, mouth and nose secretions.


Principle:

 

Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose feline herpes virus and feline calicivirus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of feline herpes virus and feline calicivirus can be detected by fluorescence PCR instrument.


Main components:
 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

[Sample requirements]

1. Sample type

Cat eye, mouth and nose secretion and other samples

 

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

 

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

The results of interpretation

 

  FAM channel (FHV) VIC/HEX channel (FCV) Result interpretation
1 - - Results were reported negative
2 + - Results were reported FHV positive
3 - + Results were reported FCV positive
4 + + Results were reported FHV,FCV positive

Note: "+" means positive, "-" means negative

 

Positive judgment value or reference interval

 

Result Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 

 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
Real Time Feline Herpes Test Kit QPCR Feline Calicivirus Test Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P030
Package:
8T/Box
Validity:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

QPCR Feline Calicivirus Test Kit

,

Feline Herpes Test Kit QPCR

,

Real Time feline calicivirus pcr

Product Description

Feline Herpes virus/Feline Caliciviral virus Detection kit

(Real time PCR probe Method)

 
Intended use:

 

This kit is suitable for the specific detection of feline herpes virus (FHV) and feline calicivirus (FCV) in cat eye, mouth and nose secretions.


Principle:

 

Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Choose feline herpes virus and feline calicivirus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of feline herpes virus and feline calicivirus can be detected by fluorescence PCR instrument.


Main components:
 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

[Sample requirements]

1. Sample type

Cat eye, mouth and nose secretion and other samples

 

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

 

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:
 

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 50℃ 20min
2 1 95℃ 2min30s
3 45 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

The results of interpretation

 

  FAM channel (FHV) VIC/HEX channel (FCV) Result interpretation
1 - - Results were reported negative
2 + - Results were reported FHV positive
3 - + Results were reported FCV positive
4 + + Results were reported FHV,FCV positive

Note: "+" means positive, "-" means negative

 

Positive judgment value or reference interval

 

Result Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 

 Operation details please check from IFU!
 

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