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DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System

DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P033
Package:
8T/Box
Validity:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

DNA Taq Feline Mycoplasma Pcr Kit

,

Feline Mycoplasma Pcr Kit Chlamydophila

,

Chlamydophila Real Time Fluorescent Pcr

Product Description

Chlamydophila / Mycoplasma felis PCR Detection kit

(Real time qPCR fluorescent probe method)


 
Intended use:

 

This kit is suitable for the specific detection of Chlamydophila felis (CF) and Mycoplasma felis (MF) in cat eye nasal and mouth secretions.


Principle:

 

This kit selection of chlamydia and mycoplasma conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to realize the detection of chlamydia and mycoplasma nucleic acids in the completely closed reaction system.


Main components:
 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Cat eye, mouth and nose secretion and other samples

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 
 DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System 0

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

The results of interpretation

 

  FAM channel (CF) VIC/HEX channel (MF) Result interpretation
1 - - Results were reported negative
2 + - Results were reported CF positive
3 - + Results were reported MF positive
4 + + Results were reported CF,MF positive

 

 

Positive judgment value or reference interval

 

Result Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 

 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-P033
Package:
8T/Box
Validity:
12 Months
Storage:
-20±5℃
Applicable:
Micgene 242/244/244 IVD PCR
LoD:
10copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

DNA Taq Feline Mycoplasma Pcr Kit

,

Feline Mycoplasma Pcr Kit Chlamydophila

,

Chlamydophila Real Time Fluorescent Pcr

Product Description

Chlamydophila / Mycoplasma felis PCR Detection kit

(Real time qPCR fluorescent probe method)


 
Intended use:

 

This kit is suitable for the specific detection of Chlamydophila felis (CF) and Mycoplasma felis (MF) in cat eye nasal and mouth secretions.


Principle:

 

This kit selection of chlamydia and mycoplasma conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was emitted and detected by fluorescence PCR instrument to realize the detection of chlamydia and mycoplasma nucleic acids in the completely closed reaction system.


Main components:
 

Component Specification
PCR reaction liquid 8 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.
 

 
Sample requirements:
 

1. Sample type

Cat eye, mouth and nose secretion and other samples

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 
 DNA Taq Feline Mycoplasma PCR Kit Chlamydophila Real Time Fluorescent Probe PCR System 0

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM and VIC/HEX are selected for detection channels.

 

The results of interpretation

 

  FAM channel (CF) VIC/HEX channel (MF) Result interpretation
1 - - Results were reported negative
2 + - Results were reported CF positive
3 - + Results were reported MF positive
4 + + Results were reported CF,MF positive

 

 

Positive judgment value or reference interval

 

Result Ct Value
Positive Ct≤38
Negative No Ct value or Ct value=0
Gray area 38<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 

 Operation details please check from IFU!
 

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