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Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit

Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W002
Package:
24T/Box
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Specimen:
Water Samples, Shrimp Seedlings
Testing Method:
NAT
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
Product Description

Infectious hypodermal and hematopoietic necrosis virus(IHHNV) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

 

 

Intended use:

 

This kit is suitable for the specific detection of IHHNV in water samples, shrimp seedlings, bait, etc., and is used for the etiological diagnosis of suspected infected persons.

 

Principle:

 

This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system.

 

Main components:

 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Sample requirements:

 

1. Sample type

Seedlings: parent, shrimp eggs, seedlings, coarse standard products

Pond: bottom mud, excrement, water body

Bait: live bait, chilled bait and non-high-temperature treated bait samples

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:

 

procedures are set as below:

Steps

Cycle number

Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃

30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Result analysis:

Automatic analysis using instrumentation

 

Quality control:

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

 

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

 

 

products
PRODUCTS DETAILS
Infectious Hypodermal Hematopoietic Necrosis Virus ( IHHNV ) Nucleic Acid Taqman QPCR Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W002
Package:
24T/Box
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Specimen:
Water Samples, Shrimp Seedlings
Testing Method:
NAT
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
Product Description

Infectious hypodermal and hematopoietic necrosis virus(IHHNV) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

 

 

Intended use:

 

This kit is suitable for the specific detection of IHHNV in water samples, shrimp seedlings, bait, etc., and is used for the etiological diagnosis of suspected infected persons.

 

Principle:

 

This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system.

 

Main components:

 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Sample requirements:

 

1. Sample type

Seedlings: parent, shrimp eggs, seedlings, coarse standard products

Pond: bottom mud, excrement, water body

Bait: live bait, chilled bait and non-high-temperature treated bait samples

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 

Using Micgene 242/244/244IVD and ABI QuantStudio5:

 

procedures are set as below:

Steps

Cycle number

Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃

30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

Result analysis:

Automatic analysis using instrumentation

 

Quality control:

Negative control: No obvious amplification curve of FAM detection channel;

Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

 

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

 

 

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