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NHPB Aquatic Pathogen Nucleic Acid Fluorescent Quantitative Pcr Taqman Test Kit

NHPB Aquatic Pathogen Nucleic Acid Fluorescent Quantitative Pcr Taqman Test Kit

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-QL-W050
Package:
Single Tube, Lyophilized Powder
Fluorescent Channel:
FAM
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Aquatic Pathogen Pcr Taqman Test Kit

,

Fluorescent Quantitative pcr Taqman Test

,

Aquatic pathogen Nucleic Acid Test Kit

Product Description

Necrotizing Hepato-pancreatitis Bacterium Nucleic Acid Detection Kit (Real time PCR, Lyophilized)


 
Intended use:

 

This kit is used for the specific detection of the Necrotizing Hepato-pancreatitis Bacterium (NHPB) in samples such as parent shrimp, shrimp fry, fish fry, bait, and processed aquatic products.
 

Principle:

 

This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes/kit
Positive control 100ul/tube
Negative control 100ul/tube
reconstitution 600ul/tube

 

Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
NHPB Aquatic Pathogen Nucleic Acid  Fluorescent Quantitative Pcr Taqman Test Kit 0

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

products
PRODUCTS DETAILS
NHPB Aquatic Pathogen Nucleic Acid Fluorescent Quantitative Pcr Taqman Test Kit
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-QL-W050
Package:
Single Tube, Lyophilized Powder
Fluorescent Channel:
FAM
Storage:
At -20±5℃, Protected From Light For 12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Mini Limit:
3copies/μL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Aquatic Pathogen Pcr Taqman Test Kit

,

Fluorescent Quantitative pcr Taqman Test

,

Aquatic pathogen Nucleic Acid Test Kit

Product Description

Necrotizing Hepato-pancreatitis Bacterium Nucleic Acid Detection Kit (Real time PCR, Lyophilized)


 
Intended use:

 

This kit is used for the specific detection of the Necrotizing Hepato-pancreatitis Bacterium (NHPB) in samples such as parent shrimp, shrimp fry, fish fry, bait, and processed aquatic products.
 

Principle:

 

This kit uses a specific fragment of Shrimp Hemocyte Iridescent Virus (SHIV) to design a primer probe, which can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorescein from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of Shrimp Hemocyte Iridescent Virus (SHIV) was detected in the completely closed reaction system.

 

Main components:
 

Component Specification
PCR reaction liquid  24 tubes/kit
Positive control 100ul/tube
Negative control 100ul/tube
reconstitution 600ul/tube

 

Note: components in different batches of kits should not be interchangeable.
 
Product picture:
 
NHPB Aquatic Pathogen Nucleic Acid  Fluorescent Quantitative Pcr Taqman Test Kit 0

 
Sample requirements:
 

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products, etc.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

 
PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃ 30s collect fluorescent

FAM is selected for detection channels.

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.


 Operation details please check from IFU!
 

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