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Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

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Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit
Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

Large Image :  Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

Product Details:
Place of Origin: Guangzhou China
Brand Name: Biokey
Certification: CE
Model Number: BIK-QL-H0022
Payment & Shipping Terms:
Minimum Order Quantity: We can produce liquid and lyophilized kits
Price: USD
Packaging Details: Carton package
Delivery Time: Depending on order quantity
Payment Terms: L/C, T/T, Western Union
Supply Ability: 100,000 per day
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Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit

Description
Package: 96 Tests/kit Expiration Date: 12 Months
Storage: -20±5℃ Applicable: Micgene 244/244 IVD（BIO-KEY）
LoD: 1×1000 Copies/ml CV: ≤5%
Highlight:

UU PCR Detection Kit

,

Ureaplasma Parvum PCR Detection Kit

,

UU STD STI Test Kit

UP+UU real-time PCR detection kit (lyophilized)
--96 tests/kit

 
Intended use:
 

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Principle:
 

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

 

UP+UU product lyophilization pacakage:

 

Biokey UP UU Real Time PCR Detection Kit Lyophilized 96 Tests/Kit 0

 
Main components:

 

No. Components BIK-QL-H0022

1

PCR Enzyme Mix (Lyophilized)

96 tests/bottle
2 UP+UU Primer-Probe Mix 100 μL/vial
3 Enzyme Mix Buffer (5×) 400 μL/vial
4 Positive Control 100 μL/tube
5 Negative Control 100 μL/tube
6 RNase-free water 1mL/tube
7 Paraffin oil 1.5mL/tube*2

  

Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.

 

Specimen requirements:
 

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

 

 

PCR Amplification condition:

 
PCR amplification according to the following procedures:
 

Step Cycle number Temperature Time
1 1 95℃ 3min
2 40 95℃ 10s
60℃ 30s collect fluorescent

 

Instrument detection channel selection: FAM and HEX/VIC.
 
Data analysis and interpretation:
 

 

FAM

(UP)

VIC/HEX

(UU)

ROX

(IC)

Interpretation test

results

1

-

-

-

Result invalid,test again
2 + - +/- UP(+)
3 - + +/- UU(+)
4 + + +/- UP and UU(+)
5 - - + Negative(-)

 

NOTE: ‘+’ is positive, ‘-’ is negative.

 

 

 Operation details please check from IFU!

Tag:

TV STD STI Test Kit,

Operation Manual RT PCR Kit,

Lyophilized RT PCR Detection Kit

Contact Details
Guangzhou BioKey Healthy Technology Co.Ltd

Contact Person: Ms. Lisa

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