Product Details:
|
Package: | 24 Tests/kit | Expiration Date: | 12 Months |
---|---|---|---|
Storage: | -20±5℃ | Applicable: | Micgene 244/244 IVD(BIO-KEY) |
LoD: | 1×1000 Copies/ml | CV: | ≤5% |
High Light: | Ureaplasma Parvum STD STI Test Kit,Ureaplasma Urealyticum PCR Detection Kit,24Tests/Kit STD STI Test Kit |
Intended use:
Principle:
UP+UU product lyophilization pacakage:
Main components:
NO. | Components |
BIK-QL-H0022S1 |
1 |
PCR Enzyme Mix(Lyophilized) |
24 tests/kit |
2 | Positive Control | 100 μL/tube |
3 | Negative Control | 100 μL/tube |
4 | RNase-free water | 1mL/tube |
5 |
Paraffin oil |
1.5mL/tube |
Specimen requirements:
1. Specimen type
Male urethral secretions or female cervical secretions.
2. Specimen Preservation
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃
PCR Amplification condition:
PCR amplification according to the following procedures:
Step | Cycle number | Temperature | Time |
1 | 1 | 95℃ | 3min |
2 | 40 | 95℃ | 10s |
60℃ | 30s collect fluorescent |
Instrument detection channel selection: FAM and HEX/VIC.
Quality control:
Negative Control: None of the FAM, VIC/HEX & Internal Control (ROX) channels have a Ct value or Ct > 40;
Positive Control: FAM, VIC/HEX & Internal Control (ROX) channels Ct ≤ 30;
The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.
Data analysis and interpretation:
FAM (UP) |
VIC/HEX (UU) |
ROX (IC) |
Interpretation test results |
|
1 |
- |
- |
- |
Result invalid,test again |
2 | + | - | +/- | UP(+) |
3 | - | + | +/- | UU(+) |
4 | + | + | +/- | UP and UU(+) |
5 | - | - | + | Negative(-) |
NOTE: ‘+’ is positive, ‘-’ is negative.
Operation details please check from IFU!
Contact Person: Lisa
Tel: +8617338314955