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Porcine Detection Kit Reproductive Respiratory Disorders Syndrome Real Time PCR

Porcine Detection Kit Reproductive Respiratory Disorders Syndrome Real Time PCR

MOQ: We produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO13485
Model Number
BIK-Q-S007
Package:
48 T/box
Storage:
Store Away From Light At -20±5℃
Validity:
12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Specimen:
Oral Swab, Serum, Viscera, Etc
CV:
≤5%
Minimum Order Quantity:
We produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Supply Ability:
100,000 per day
High Light:

Porcine Detection Kit NA PCR

,

PRRSV Real Time PCR Kits

,

PRRSV Porcine Detection Kit

Product Description

Porcine reproductive and respiratory disorders syndrome(NA) Nucleic Acid Detection Kits (PCR-Fluorescent Probe Method)

 

 

 

Intended use:

 

This kit is used for the diagnosis, monitoring and epidemiological investigation of classical swine fever, and is suitable for the specific detection of porcine reproductive and respiratory syndrome virus (PRRSV) in oral swab, serum and viscera samples.

 

Principle:

 

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Selection of porcine reproductive and respiratory syndrome virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of PORcine reproductive and respiratory syndrome virus was detected by fluorescence PCR apparatus in a completely closed reaction system.

 

Reagent kits main components:

 

Component Specification
PCR reaction liquid 48 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit:

The detection limit of this kit is 3 copies/μL.

3. Precision:

Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

 

Sample requirements:

 

1. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

2. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

 

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

products
PRODUCTS DETAILS
Porcine Detection Kit Reproductive Respiratory Disorders Syndrome Real Time PCR
MOQ: We produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO13485
Model Number
BIK-Q-S007
Package:
48 T/box
Storage:
Store Away From Light At -20±5℃
Validity:
12 Months
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
Specimen:
Oral Swab, Serum, Viscera, Etc
CV:
≤5%
Minimum Order Quantity:
We produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Supply Ability:
100,000 per day
High Light

Porcine Detection Kit NA PCR

,

PRRSV Real Time PCR Kits

,

PRRSV Porcine Detection Kit

Product Description

Porcine reproductive and respiratory disorders syndrome(NA) Nucleic Acid Detection Kits (PCR-Fluorescent Probe Method)

 

 

 

Intended use:

 

This kit is used for the diagnosis, monitoring and epidemiological investigation of classical swine fever, and is suitable for the specific detection of porcine reproductive and respiratory syndrome virus (PRRSV) in oral swab, serum and viscera samples.

 

Principle:

 

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Selection of porcine reproductive and respiratory syndrome virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of PORcine reproductive and respiratory syndrome virus was detected by fluorescence PCR apparatus in a completely closed reaction system.

 

Reagent kits main components:

 

Component Specification
PCR reaction liquid 48 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Product performance index:

 

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit:

The detection limit of this kit is 3 copies/μL.

3. Precision:

Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

 

Sample requirements:

 

1. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

2. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

 

 

 

The results of interpretation:

 

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

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