|Package:||96 T/kit (carton)||Specimen Preservation:||2 Weeks Ar 4-8℃,3 Months At -20± 5℃ And For A Long Time Below -70℃.|
|Applicable:||Micgene 244/244 IVD(BIO-KEY)||Detection Limit:||500 Copies/mL|
Hpv Real Time Pcr Kit High Risk,
Real Time Hpv Real Time Pcr Kit,
Micgene Nucleic Acid DNA Detection Kit
Real Time quantitative PCR High risk Human Papillomavirus ( HPV ) Nucleic Acid DNA Detection Kit
This kit, adopting Real-Time PCR-Fluorescence technology, is used for qualitative detection of HPV 16+ 18. It can be used for the laboratory diagnosis and monitoring of the virus infection. The test results are only for clinical reference, not for case confirmation or exclusion.
The specific primers and specific fluorescent probes are designed for the conserved region of HPV 16+ 18. The PCR reaction solution, and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of HPV 16+ 18 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of the nucleic acid extraction is qualified.
|1||PCR Enzyme Mix (Lyophilized)||96 tests/bottle|
|2||HPV Primer-Probe Mix||100 μL/vial|
|3||Enzyme Mix Buffer (5×)||400 μL/vial|
|4||Positive Control||100 μL/tube|
|5||Negative Control||100 μL/tube|
Note: Do not mix the components from different batches for detection. The Positive Control of HPV and internal control were constructed artificially, and they were not infectious.
The following sample results are possible:
|Ct value||Result Analysis|
|3#||38~40||Re-test; if it is still 38~40, report as 2#|
Reaction mix preparation :
Refer to one of the table below to prepare the reaction mix:
|Resuspended master mix||14 μL|
|HPV Primer-Probe Mix||1 μL|
|Total volume||15 μL|
※Multiply the numbers according to the number of tests.
Interpretation of test results:
|FAM (HPV16)||VIC/HEX (HPV18)||ROX(IC)||Interpretation test results|
|1||-||-||-||Result invalid, test again|
|4||+||+||+/-||HPV16 and HPV18(+)|
NOTE: ‘+ ’ is positive, ‘- ’ is negative.
Operation details please check from IFU
Contact Person: Ms. Lisa