|Storage:||-20±5℃||Applicable:||Micgene 242/244/244 IVD PCR|
ISO 13485 One Step Real Time Pcr Kit,
ISO 13485 Giardia Pcr Test kit,
Giardia One Step Real Time Pcr Kit T Foetus
T.foetus/Giardia(TF/GD) nucleic acid testing kit
(Real time PCR Method)
This kit is suitable for the specific detection of T.foetus (TF) and giardia (GD) in fetal stool samples.
This kit selection and the drop of the T.foetus/Giardia(TF/GD) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acids of trichomonas and giardia in the fetus were detected in the completely closed reaction system.
|PCR reaction liquid||8 tubes|
|Positive control||1 tube|
|Negative control||1 tube|
1. Sample type
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Using Micgene 242/244/244IVD and ABI QuantStudio5:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
|60℃||30s collect fluorescent|
FAM and VIC/HEX are selected for detection channels.
The results of interpretation
|FAM channel (CF)||VIC/HEX channel (MF)||Result interpretation|
|1||-||-||Results were reported negative|
|2||+||-||Results were reported TF positive|
|3||-||+||Results were reported GD positive|
|4||+||+||Results were reported TF,GD positive|
Positive judgment value or reference interval
|Negative||No Ct value or Ct value=0|
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!
Contact Person: Ms. Lisa