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Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit

Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit

MOQ: We can produce liquid and lyophilized PCR probe kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W001
Package:
24T/Box
Specimen:
Tissue Samples From Prawn
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized PCR probe kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Prawn PCR WSSV Rapid Test Kit

,

White Spot Syndrome Virus WSSV Test Kit ISO

,

Prawn Baculovirus PCR Kit

Product Description

White spot syndrome virus WSSV Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

 

 

Intended use:

 

This kit is suitable for the detection of Shrimp white spot syndrome virus (WSSV) in shrimp samples according to the different size or infection stage of different tissue samples, used for the auxiliary diagnosis of shrimp white spot syndrome virus (WSSV) infection.

 

Principle:

 

This kit uses a specific fragment of white spot syndrome virus (WSSV) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclization activity of Taq enzyme cuts the 5 '-terminal fluoromeric group from the probe, making it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of white spot syndrome virus (WSSV) was detected in the completely closed reaction system.

 

Biokey Aquaculture PCR testing solution.pdf

 

Main components:

 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Sample requirements:

 

1. Sample type

Tissue samples from prawn.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3.Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

4. Sample extraction&purification processing with professional machine below:

Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit 0

Using Micgene 242/244/244IVD and ABI QuantStudio5:

 

procedures are set as below:

Steps

Cycle number

Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃

30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

 

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

procedures are set as below:

 

Steps Cycle number Temperature Time
1 1 95℃ 3min
2 10 95℃ 10s
60℃ 30s
3 35 95℃ 10s
60℃ 30sCollect the fluorescent

 

FAM was selected for detection channel.

 

The results of interpretation:

 

Results

Ct Value
Positive Ct≤35
Negative No Ct value or Ct value≥35
Gray area 30<Ct<35

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 35 is considered as positive, and no Ct value or Ct value ≥35 is considered as negative.

 

Details please check from IFU!

 

We offer the full PCR testing solution to support the shrimp disease detection and monitoring on site.

 

Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit 1

products
PRODUCTS DETAILS
Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit
MOQ: We can produce liquid and lyophilized PCR probe kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485
Model Number
BIK-Q-W001
Package:
24T/Box
Specimen:
Tissue Samples From Prawn
Validity:
12 Months
Storage:
Store Away From Direct Light At -20±5℃
Applicable:
Micgene 242 / 244 、ABI QuantStudio5
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized PCR probe kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Prawn PCR WSSV Rapid Test Kit

,

White Spot Syndrome Virus WSSV Test Kit ISO

,

Prawn Baculovirus PCR Kit

Product Description

White spot syndrome virus WSSV Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

 

 

Intended use:

 

This kit is suitable for the detection of Shrimp white spot syndrome virus (WSSV) in shrimp samples according to the different size or infection stage of different tissue samples, used for the auxiliary diagnosis of shrimp white spot syndrome virus (WSSV) infection.

 

Principle:

 

This kit uses a specific fragment of white spot syndrome virus (WSSV) to design a primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclization activity of Taq enzyme cuts the 5 '-terminal fluoromeric group from the probe, making it free in the reaction system. Thus, the 3 '-end quenched group was separated, and the fluorescence was emitted, which was detected by fluorescence PCR instrument, and the nucleic acid of white spot syndrome virus (WSSV) was detected in the completely closed reaction system.

 

Biokey Aquaculture PCR testing solution.pdf

 

Main components:

 

Component Specification
PCR reaction liquid  24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

 

Sample requirements:

 

1. Sample type

Tissue samples from prawn.

2. Transport

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3.Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

4. Sample extraction&purification processing with professional machine below:

Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit 0

Using Micgene 242/244/244IVD and ABI QuantStudio5:

 

procedures are set as below:

Steps

Cycle number

Temperature Time
1 1 95℃ 3min
2 45 95℃ 10s
60℃

30sCollect the fluorescent

FAM was selected for detection channel.

 

The results of interpretation

Results Ct Value
Positive Ct≤45
Negative No Ct value or Ct value≥45
Gray area 40<Ct≤45

 

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

 

procedures are set as below:

 

Steps Cycle number Temperature Time
1 1 95℃ 3min
2 10 95℃ 10s
60℃ 30s
3 35 95℃ 10s
60℃ 30sCollect the fluorescent

 

FAM was selected for detection channel.

 

The results of interpretation:

 

Results

Ct Value
Positive Ct≤35
Negative No Ct value or Ct value≥35
Gray area 30<Ct<35

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 35 is considered as positive, and no Ct value or Ct value ≥35 is considered as negative.

 

Details please check from IFU!

 

We offer the full PCR testing solution to support the shrimp disease detection and monitoring on site.

 

Vannamei shrimp White Spot Syndrome Virus WSSV Rapid Test Kit Prawn Baculovirus PCR Kit 1

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