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Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized

MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485,CE
Model Number
BIK-QL-H003S
Package:
48 T/kit (single Tube)
Specimen:
Human Throat Swabs, Herpes Fluid Samples, Stool Samples, And Anal Swab Samples.
Specimen Preservation:
Be Stored For 3 Months At -20±5℃ And For A Long Time Below -70℃
Applicable:
Micgene 244/244 IVD(BIO-KEY)
Detection Limit:
500 Copies/mL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light:

Biokey Real Time Pcr Diagnostic Lyophilized

,

Biokey Real Time Pcr Diagnostic Lyophilized

,

Lyophilized RT-PCR Detection Kit micgene

Product Description

Real Time Diagnostics PCR Enterovirus 71 ( EV71 ) RT-PCR Detection Kit ( Lyophilized )

 

Intended use:
 

By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Test Principle:


The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophi...


Product components:

 

No. Components BIK-QL-H003S
1 PCR Enzyme Mix (Lyophilized) 48 tests/kit
2 EV71 Primer-Probe Mix /
3 Enzyme Mix Buffer (5×) /
4 Positive Control 100 μL/tube
5 Negative Control 100 μL/tube
6 RNase-free water 1mL/tube
7 Paraffin oil 1.5mL/tube


Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.

 

Compatible instruments:

 

The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).

 

Reaction Mix Preparation:

 

Refer to one of the table below to prepare the reaction mix:

 

1×volume required

Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

Multiply the numbers according to the number of tests.

 

 

PCR amplification condition:

 

PCR amplification according to the following procedure:

 

Step Cycle Temperature Time
1 1 50℃ 10 min
2 1 94℃ 3 min

 

3

 

40

94℃ 10 s
60℃

30 s

(Collection of fluorescence)


Select the detection channels as following:

a) Select FAM channels to detect EV71.

b) Select HEX/VIC channel to detect internal control.

 

Reaction Mix Preparation:

 

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

Multiply the numbers according to the number of tests.

 

Product picture:

 

 

Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit  Lyophilized 0

 

 Operation details please check from IFU

products
PRODUCTS DETAILS
Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit Lyophilized
MOQ: We can produce liquid and lyophilized kits
Price: USD
Standard Packaging: Carton package
Delivery Period: Depending on order quantity
Payment Method: L/C, T/T, Western Union
Supply Capacity: 100,000 per day
Detail Information
Place of Origin
Guangzhou China
Brand Name
Biokey
Certification
ISO 13485,CE
Model Number
BIK-QL-H003S
Package:
48 T/kit (single Tube)
Specimen:
Human Throat Swabs, Herpes Fluid Samples, Stool Samples, And Anal Swab Samples.
Specimen Preservation:
Be Stored For 3 Months At -20±5℃ And For A Long Time Below -70℃
Applicable:
Micgene 244/244 IVD(BIO-KEY)
Detection Limit:
500 Copies/mL
CV:
≤5%
Minimum Order Quantity:
We can produce liquid and lyophilized kits
Price:
USD
Packaging Details:
Carton package
Delivery Time:
Depending on order quantity
Payment Terms:
L/C, T/T, Western Union
Supply Ability:
100,000 per day
High Light

Biokey Real Time Pcr Diagnostic Lyophilized

,

Biokey Real Time Pcr Diagnostic Lyophilized

,

Lyophilized RT-PCR Detection Kit micgene

Product Description

Real Time Diagnostics PCR Enterovirus 71 ( EV71 ) RT-PCR Detection Kit ( Lyophilized )

 

Intended use:
 

By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection. The test results are only for clinical reference, not for case confirmation or exclusion.

 

Test Principle:


The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophi...


Product components:

 

No. Components BIK-QL-H003S
1 PCR Enzyme Mix (Lyophilized) 48 tests/kit
2 EV71 Primer-Probe Mix /
3 Enzyme Mix Buffer (5×) /
4 Positive Control 100 μL/tube
5 Negative Control 100 μL/tube
6 RNase-free water 1mL/tube
7 Paraffin oil 1.5mL/tube


Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.

 

Compatible instruments:

 

The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).

 

Reaction Mix Preparation:

 

Refer to one of the table below to prepare the reaction mix:

 

1×volume required

Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

Multiply the numbers according to the number of tests.

 

 

PCR amplification condition:

 

PCR amplification according to the following procedure:

 

Step Cycle Temperature Time
1 1 50℃ 10 min
2 1 94℃ 3 min

 

3

 

40

94℃ 10 s
60℃

30 s

(Collection of fluorescence)


Select the detection channels as following:

a) Select FAM channels to detect EV71.

b) Select HEX/VIC channel to detect internal control.

 

Reaction Mix Preparation:

 

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

Multiply the numbers according to the number of tests.

 

Product picture:

 

 

Enterovirus EV71 Real Time PCR Diagnostic RT-PCR Detection Kit  Lyophilized 0

 

 Operation details please check from IFU

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